LOCUS       NC_001747               5987 bp ss-RNA     linear   VRL 25-JAN-2016
DEFINITION  Potato leafroll virus, complete genome.
ACCESSION   NC_001747
VERSION     NC_001747.1  gi:9629160
KEYWORDS    RefSeq; coat protein; genome; polymerase; unidentified reading
            frame.
SOURCE      Potato leafroll virus
  ORGANISM  Potato leafroll virus
            Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA
            stage; Luteoviridae; Polerovirus.
REFERENCE   1
  AUTHORS   Prufer, D., Tacke, E., Schmitz, J., Kull, B., Kaufmann, A., Rohde,
            W.
  TITLE     Ribosomal frameshifting in plants: a novel signal directs the -1
            frameshift in the synthesis of the putative viral replicase of
            potato leafroll luteovirus
  JOURNAL   EMBO J. 11 (3), 1111-1117 (1992)
  PUBMED    1547775
REFERENCE   2
  AUTHORS   Mayo, M.A., Robinson, D.J., Jolly, C.A., Hyman, L.
  TITLE     Nucleotide sequence of potato leafroll luteovirus RNA
  JOURNAL   J. Gen. Virol. 70 (Pt 5), 1037-1051 (1989)
  PUBMED    2732710
REFERENCE   3
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2000) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   4
  AUTHORS   Mayo, M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-1989) Mayo M.A., Scottish Crop Research
            Institute, Invergowrie, Dundee, DD2 5DA, U K
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            D00530.; COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Potato leafroll virus"
                     /mol_type="genomic RNA"
                     /db_xref="taxon:12045"
     gene            175..918
                     /locus_tag="PLRVgp1"
                     /db_xref="GeneID:1493890"
     CDS             175..918
                     /locus_tag="PLRVgp1"
                     /note="ORF 1"
                     /codon_start=1
                     /transl_table=1
                     /product="P0 protein"
                     /protein_id="NP_056746.1"
                     /db_xref="GI:9629161"
                     /db_xref="UniProtKB/Swiss-Prot:P17518"
                     /db_xref="GeneID:1493890"
                     /translation="MIVLTQSGTLLFDQRFKLSKFLFVVIATGFPLLLQQASLIYGYN
                     HEQIYRICRSFLHVLPLLNCKRGRISTSGLQLPRHLHYECLEWGLLCGTHPAIQIVGL
                     TIVIKLDDPTTAAAYRSELLRVSSSSYIQNAAGLSNGWGHDMEAFVRNAICLLELRER
                     SIPQSGLRDLMGNYQHLVRSLLDACKVDHFVPLDFQHRSLMLNFARLYNQLDLQGRAK
                     SFRALTGFPVYVPSEDYLEGSFLQKELQE"
     gene            308..3495
                     /locus_tag="PLRVgp2"
                     /db_xref="GeneID:1493893"
     CDS             join(308..1774, 1774..3495)
                     /locus_tag="PLRVgp2"
                     /experiment="EXISTENCE:site-directed mutagenesis[1547775]"
                     /exception="artificial frameshift"
                     /note="ORF 2b"
                     /codon_start=1
                     /transl_table=1
                     /product="RNA-dependent RNA polymerase"
                     /protein_id="NP_056748.3"
                     /db_xref="GI:445110392"
                     /db_xref="UniProtKB/Swiss-Prot:P17520"
                     /db_xref="GeneID:1493893"
                     /translation="MNRFTAYAALFFMFSLCSTAKEAGFLHPAFNFRGTSTMSASSGD
                     YSAAPTPLYKSWALPSSLNLTTQPPPPLTDRSYYELVQALTSKMRLDCQTVGDMTWRH
                     LSEMLFASWNSVKEVSLKAASVTLWAIINIWFGLYWTLARLITLFLWTFSIEALCLIL
                     LGCITSLIYKGALSLSEHLPVFLFMSPLKIIWRAAFSKRNYKNERAVEGYKGFSVPQK
                     PPKSAVIELQHENGSHLGYANCIRLYSGENALVTAEHCLEGAFATSLKTGNRIPMSTF
                     FPIFKSARNDISILVGPPNWEGLLSVKGAHFITADKIGKGPASFYTLEKGEWMCHSAT
                     IDGAHHQFVSVLCNTGPGYSGTGFWSSKNLLGVLKGFPLEEECNYNVMSVIPSIPGIT
                     SPNYVFESTAVKGRVFSDEAVKELEREASEAVKKLARFKSLTDKNWADDYDSDEDYGL
                     EREAATNAPAEKTAQTNSAEKTAPSTSAEKTALTNKPLNWASGTVRQNKRQLRHPRRR
                     YKRTTNGQNGRTDHHSYGGENQSLGDRGEDSEQGVSESPAEAQTKEARKAWREEQAKQ
                     FTSYFNAIYKWGAQEGGCPPGFRKCGHIPRYYHPRTRGETQWGQKLCQVHPELADKTA
                     GFGWPKAGSEAELQSLNLQAARWLQRAESATIPGAEARKRVIEKTVEAYRNCVTNAPL
                     CSLKSKLDWAGFQQDIREAVQSLELDAGVGIPYIAYGLPAHRGWVEDHKLLPVLTQLT
                     FDRLQKMSEASFEDMSAEELVQEGLCDPIRLFVKGEPHKQSKLDEGRYRLIMSVSLVD
                     QLVARVLFQNQNKREISLWRSVPSKPGFGLSTDTQTAEFLECLQKVSGAPSVEELCAN
                     HKEYTRPTDCSGFDWSVAYWMLEDDMEVRNRLTFNNTQLTKRLRAAWLKCIGNSVLCL
                     SDGTLLAQTVPGVQKSGSYNTSSSNSRIRVMAAYHCGADWAMAMGDDALEAPNSDLEE
                     YKTLGFKVEVGRELEFCSHIFRNPTLAVPVNTNKMLYKLIHGYNPECGNPEVIQNYLA
                     AVFSVLQELRHDRELVAKLHQWLVPSATTKEH"
     gene            308..2227
                     /locus_tag="PLRVgp3"
                     /db_xref="GeneID:1493894"
     CDS             308..2227
                     /locus_tag="PLRVgp3"
                     /note="ORF 2a"
                     /codon_start=1
                     /transl_table=1
                     /product="P1 protein"
                     /protein_id="NP_056747.1"
                     /db_xref="GI:9629162"
                     /db_xref="UniProtKB/Swiss-Prot:P17519"
                     /db_xref="GeneID:1493894"
                     /translation="MNRFTAYAALFFMFSLCSTAKEAGFLHPAFNFRGTSTMSASSGD
                     YSAAPTPLYKSWALPSSLNLTTQPPPPLTDRSYYELVQALTSKMRLDCQTVGDMTWRH
                     LSEMLFASWNSVKEVSLKAASVTLWAIINIWFGLYWTLARLITLFLWTFSIEALCLIL
                     LGCITSLIYKGALSLSEHLPVFLFMSPLKIIWRAAFSKRNYKNERAVEGYKGFSVPQK
                     PPKSAVIELQHENGSHLGYANCIRLYSGENALVTAEHCLEGAFATSLKTGNRIPMSTF
                     FPIFKSARNDISILVGPPNWEGLLSVKGAHFITADKIGKGPASFYTLEKGEWMCHSAT
                     IDGAHHQFVSVLCNTGPGYSGTGFWSSKNLLGVLKGFPLEEECNYNVMSVIPSIPGIT
                     SPNYVFESTAVKGRVFSDEAVKELEREASEAVKKLARFKSLTDKNWADDYDSDEDYGL
                     EREAATNAPAEKTAQTNSAEKTAPSTSAEKTALTNKPLNGQAAPSAKTNGNSDIPDAA
                     TSAPPMDKMVEQIITAMVGRINLSEIEEKIVSRVSQKALQKPKQKKRGRRGGKNKQNS
                     LPPTSTQSTSGAPKKEAAPQASGSAGTSRATTTPAPEAKPSGGKNSAKFTPSWRIKQQ
                     DSAGQKPDLKLNSKA"
     Polymorphism    479
                     /locus_tag="PLRVgp1"
                     /note="t in cloned cDNA from first RNA preparation (first
                     clones); c in cloned cDNA from second RNA preparation
                     (second clones)"
     Polymorphism    521
                     /locus_tag="PLRVgp1"
                     /note="c in first clones; t in second clones"
     Polymorphism    571
                     /locus_tag="PLRVgp1"
                     /note="c in first clones; t in second clones"
     Polymorphism    937
                     /locus_tag="PLRVgp3"
                     /note="c in first clones; t in second clones"
     Polymorphism    1474
                     /locus_tag="PLRVgp3"
                     /note="g in first clones; a in second clones"
     Polymorphism    1611
                     /locus_tag="PLRVgp3"
                     /note="a in first clones; g in second clones"
     gene            1632..1757
                     /gene="Rap1"
                     /locus_tag="RLRVgp5"
                     /db_xref="GeneID:12978168"
     CDS             1632..1757
                     /gene="Rap1"
                     /locus_tag="RLRVgp5"
                     /note="Replication-associated protein has been added by
                     NCBI Staff with the kind help of Dr. David
                     Karlin(Department of Zoology, University of Oxford, UK)."
                     /codon_start=1
                     /transl_table=1
                     /product="Replication-associated protein"
                     /protein_id="YP_006355442.1"
                     /db_xref="GI:388483858"
                     /db_xref="GeneID:12978168"
                     /translation="MTPMRITVWRERLQQMRPQRKLLKQTQQRRLLHQLQQRKLL"
     Polymorphism    1755
                     /gene="Rap1"
                     /locus_tag="RLRVgp5"
                     /note="t in first clones; c in second clones"
     Polymorphism    1774
                     /locus_tag="PLRVgp3"
                     /note="t in first clones; c in second clones"
     Polymorphism    1969
                     /locus_tag="PLRVgp3"
                     /note="g in first clones; a in second clones"
     Polymorphism    2004
                     /locus_tag="PLRVgp3"
                     /note="g in first clones; a in second clones"
     Polymorphism    2020
                     /locus_tag="PLRVgp3"
                     /note="a in first clones; g in second clones"
     Polymorphism    2031
                     /locus_tag="PLRVgp3"
                     /note="c in first clones; t in second clones"
     Polymorphism    2083
                     /locus_tag="PLRVgp3"
                     /note="c in first clones; a in second clones"
     Polymorphism    2085
                     /locus_tag="PLRVgp3"
                     /note="c in first clones; t in second clones"
     Polymorphism    2118
                     /locus_tag="PLRVgp3"
                     /note="c in first clones; a in second clones"
     Polymorphism    2125
                     /locus_tag="PLRVgp3"
                     /note="c in first clones; a in second clones"
     Polymorphism    2169
                     /locus_tag="PLRVgp3"
                     /note="t in first clones; g in second clones"
     Polymorphism    2470
                     /locus_tag="PLRVgp2"
                     /note="g in first clones; a in second clones"
     Polymorphism    2565
                     /locus_tag="PLRVgp2"
                     /note="g in first clones; a in second clones"
     Polymorphism    2583
                     /locus_tag="PLRVgp2"
                     /note="g in first clones; a in second clones"
     Polymorphism    2586
                     /locus_tag="PLRVgp2"
                     /note="a in first clones; g in second clones"
     Polymorphism    2649
                     /locus_tag="PLRVgp2"
                     /note="c in first clones; t in second clones"
     Polymorphism    2898
                     /locus_tag="PLRVgp2"
                     /note="t in first clones; c in second clones"
     Polymorphism    3000
                     /locus_tag="PLRVgp2"
                     /note="t in first clones; c in second clones"
     Polymorphism    3009
                     /locus_tag="PLRVgp2"
                     /note="t in first clones; c in second clones"
     Polymorphism    3186
                     /locus_tag="PLRVgp2"
                     /note="t in first clones; c in second clones"
     Polymorphism    3282
                     /locus_tag="PLRVgp2"
                     /note="c in first clones; t in second clones"
     Polymorphism    3525
                     /note="a in first clones; g in second clones"
     Polymorphism    3572
                     /note="t in first clones; a in second clones"
     gene            3575..3712
                     /gene="ORF3a"
                     /locus_tag="PLRVgp7"
                     /db_xref="GeneID:26196468"
     CDS             3575..3712
                     /gene="ORF3a"
                     /locus_tag="PLRVgp7"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="p3a; essential for viral movement; Non-AUG
                     initiation (AUA initiation); non-AUG codon is translated
                     by initiator-tRNA-Met giving N-terminal Met. ORF3a in
                     Luteovirus and Polerovirus genera was predicted by
                     statistical analysis of sequence variation in large set of
                     aligned sequences. Functional analysis of protein 3a was
                     conducted with Turnip yellows virus. ORF3a was translated
                     in vitro, and confirmed by immunological assay in infected
                     protoplasts and in agroinoculated plants. Mutations that
                     prevent expression of protein 3a prevent virus systemic
                     infection (long-distance movement in plants). PMID
                     25946037"
                     /codon_start=1
                     /transl_except="(pos:3575..3577, aa:Met)"
                     /transl_table=1
                     /product="protein 3a"
                     /protein_id="YP_009179365.2"
                     /db_xref="GI:982725828"
                     /db_xref="GeneID:26196468"
                     /translation="MDYKFLAGFALGFSSAIPFSVAGLYFVYLKISSHVRSIVNEYGR
                     G"
     gene            3693..5846
                     /locus_tag="PLRVgp4"
                     /db_xref="GeneID:1493889"
     CDS             3693..5846
                     /locus_tag="PLRVgp4"
                     /note="ORF 5"
                     /codon_start=1
                     /transl_except="(pos:4317..4319, aa:OTHER)"
                     /transl_table=1
                     /product="CP read-through protein"
                     /protein_id="NP_056751.2"
                     /db_xref="GI:21040163"
                     /db_xref="UniProtKB/TrEMBL:Q84813"
                     /db_xref="GeneID:1493889"
                     /translation="MSTVVVKGNVNGGVQQPRMRRRQSLRRRANRVQPVVMVTAPGQP
                     RRRRRRRGGNRRSRRTGVPRGRGSSETFVFTKDNLVGNTQGSFTFGPSLSDCPAFKDG
                     ILKAYHEYKITSILLQFVSEASSTSSGSIAYELDPHCKVSSLQSYVNKFQITKGGAKT
                     YQARMINGVEWHDSSEDQCRILWKGNGKSSDSAGSFRVTIKVALQNPKXVDSGSEPSP
                     SPQPTPTPTPQKHERFIAYVGIPMLTIQARENDDQIILGSLGSQRMKYIEDENQNYTK
                     FSSEYYSQSSMQAVPMYYFNVPKGQWSVDISCEGYQPTSSTSDPNRGRSDGMIAYSNA
                     DSDYWNVGEADGVKISKLRNDNTYRQGHPELEINSCHFREGQLLERDATISFHVEAPT
                     DGRFFLVGPAIQKTAKYNYTISYGDWTDRDMELGLITVVLDEHLEGTGSANRVRRPPR
                     EGHTYMASPHEPEGKPVGNKPRDETPIQTQERQPDQTPSDDVSDAGSVNSGGPTESLR
                     LEFGVNSDSTYDATVDGTDWPRIPPPRHPPEPRVSGNSRTVTDFSSKADLLENWDAEH
                     FDPGYSKEDVAAATIIAHGSIQDGRSMLEKREENVKNKTSSWKPPSLKAVSPAIAKLR
                     SIRKSQPLEGGTLNKDATDGVSSIGSGSLTGGTLKRKATIEERLLQTLTTEQRLWYEN
                     FKKTNPPAATQWLFEYQPPPQVDRNIAEKPFQGRK"
     gene            3693..4319
                     /locus_tag="PLRVgp5"
                     /db_xref="GeneID:1493891"
     CDS             3693..4319
                     /locus_tag="PLRVgp5"
                     /note="ORF 3"
                     /codon_start=1
                     /transl_table=1
                     /product="coat protein"
                     /protein_id="NP_056749.1"
                     /db_xref="GI:9629164"
                     /db_xref="UniProtKB/Swiss-Prot:P17522"
                     /db_xref="GeneID:1493891"
                     /translation="MSTVVVKGNVNGGVQQPRMRRRQSLRRRANRVQPVVMVTAPGQP
                     RRRRRRRGGNRRSRRTGVPRGRGSSETFVFTKDNLVGNTQGSFTFGPSLSDCPAFKDG
                     ILKAYHEYKITSILLQFVSEASSTSSGSIAYELDPHCKVSSLQSYVNKFQITKGGAKT
                     YQARMINGVEWHDSSEDQCRILWKGNGKSSDSAGSFRVTIKVALQNPK"
     gene            3718..4188
                     /locus_tag="PLRVgp6"
                     /db_xref="GeneID:1493892"
     CDS             3718..4188
                     /locus_tag="PLRVgp6"
                     /note="ORF 4"
                     /codon_start=1
                     /transl_table=1
                     /product="putative genome-linked protein"
                     /protein_id="NP_056750.1"
                     /db_xref="GI:9629165"
                     /db_xref="UniProtKB/Swiss-Prot:P17524"
                     /db_xref="GeneID:1493892"
                     /translation="MSMVVYNNQECEEGNPFAGALTEFSQWLWSRPLGNPGAEDAEEE
                     AIAAQEELEFPEDEAQARHSCLQRTTSWATPKEVSPSGRVYQTVRHSRMEYSRPTMSI
                     RSQASYFSSSARPLPPPPVPSLMSWTPIAKYHPSSPTSTSSKLRRAAPKLIKRG"
     Polymorphism    3748
                     /locus_tag="PLRVgp6"
                     /note="t in first clones; g in second clones"
     Polymorphism    3930
                     /locus_tag="PLRVgp6"
                     /note="g in first clones; a in second clones"
     Polymorphism    3939
                     /locus_tag="PLRVgp6"
                     /note="a in first clones; t in second clones"
     Polymorphism    4501
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; g in second clones"
     Polymorphism    4510
                     /locus_tag="PLRVgp4"
                     /note="g in first clones; c in second clones"
     Polymorphism    4511
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; a in second clones"
     Polymorphism    4697
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; c in second clones"
     Polymorphism    4817
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; c in second clones"
     Polymorphism    4883
                     /locus_tag="PLRVgp4"
                     /note="c in first clones; t in second clones"
     Polymorphism    4886
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; c in second clones"
     Polymorphism    4895
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; c in second clones"
     Polymorphism    4898
                     /locus_tag="PLRVgp4"
                     /note="c in first clones; t in second clones"
     Polymorphism    5038
                     /locus_tag="PLRVgp4"
                     /note="c in first clones; t in second clones"
     Polymorphism    5042
                     /locus_tag="PLRVgp4"
                     /note="g in first clones; a in second clones"
     Polymorphism    5071
                     /locus_tag="PLRVgp4"
                     /note="a in first clones; g in second clones"
     Polymorphism    5199
                     /locus_tag="PLRVgp4"
                     /note="c in first clones; t in second clones"
     Polymorphism    5215
                     /locus_tag="PLRVgp4"
                     /note="g in first clones; a in second clones"
     Polymorphism    5247
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; c in second clones"
     Polymorphism    5339
                     /locus_tag="PLRVgp4"
                     /note="c in first clones; t in second clones"
     Polymorphism    5341
                     /locus_tag="PLRVgp4"
                     /note="c in first clones; t in second clones"
     Polymorphism    5352
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; c in second clones"
     Polymorphism    5393
                     /locus_tag="PLRVgp4"
                     /note="c in first clones; t in second clones"
     Polymorphism    5426
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; c in second clones"
     Polymorphism    5477
                     /locus_tag="PLRVgp4"
                     /note="c in first clones; t in second clones"
     Polymorphism    5490
                     /locus_tag="PLRVgp4"
                     /note="a in first clones; g in second clones"
     Polymorphism    5495
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; c in second clones"
     Polymorphism    5511
                     /locus_tag="PLRVgp4"
                     /note="a in first clones; t in second clones"
     Polymorphism    5741
                     /locus_tag="PLRVgp4"
                     /note="t in first clones; g in second clones"
     Polymorphism    5767
                     /locus_tag="PLRVgp4"
                     /note="c in first clones; t in second clones"
ORIGIN      
        1 ctttatgata ctatgcaaat tgtgctacca gaaatccgta cagtatgcgt aggattagcc
       61 gcttcaatgg gatctgttat cctggtcgga ggagaaatta ccaaacgtct agcattcccg
      121 agaaattgca gctttagcgc ataaactcta cactcattgc aaacgttata gcatatgatt
      181 gtattgaccc agtctggaac cttgcttttt gaccaaagat ttaaactctc aaagtttctc
      241 ttcgttgtca ttgcaacagg ctttcctctt ctcctgcagc aagcgagctt aatttacggc
      301 tataatcatg aacagattta ccgcatatgc cgctcttttc ttcatgttct ccctttgctc
      361 aactgcaaaa gaggcaggat ttctacatcc ggccttcaac ttccgaggca cctccactat
      421 gagtgcctcg agtggggatt actctgcggc acccaccccg ctatacaaat cgtgggcctt
      481 accatcgtca ttaaacttga cgacccaacc accgccgccg cttacagatc ggagctacta
      541 cgagttagtt caagctctta catccaaaat gcggctggat tgtcaaacgg ttggggacat
      601 gacatggagg catttgtcag aaatgctatt tgcctcctgg aactccgtga aagaagtatc
      661 cctcaaagcg gcctccgtga ccttatgggc aattatcaac atttggttcg gtctctattg
      721 gacgcttgca aggttgatca ctttgttcct ctggactttc agcatagaag ccttatgctt
      781 aattttgctc ggttgtataa ccagcttgat ctacaagggc gcgctaagtc tttcagagca
      841 cttaccggtt ttcctgttta tgtcccctct gaagattatt tggagggcag ctttctccaa
      901 aaggaattac aagaatgaga gggctgtgga aggatacaaa gggttttcgg tcccacagaa
      961 accgccaaag tctgccgtaa ttgaactaca acatgaaaac ggcagccatc tcgggtacgc
     1021 gaactgcatt cgcttgtaca gtggagagaa cgccttggtg acagctgaac actgtctaga
     1081 aggcgctttc gcaacgtcgt tgaaaactgg aaacaggatt ccgatgtcga ctttctttcc
     1141 cattttcaaa agtgcccgta atgatatctc catactagta ggtccaccca actgggaagg
     1201 tctactatca gtcaaaggag ctcatttcat tacagctgac aaaatcggca aaggtcctgc
     1261 ctctttctac actcttgaga aaggggagtg gatgtgccat agtgccacca tagatggagc
     1321 ccatcaccag ttcgtgtctg ttttatgcaa cactggaccc ggatattccg gaacagggtt
     1381 ttggtcttca aagaatctgc ttggtgtgct taaaggcttc ccactggaag aggagtgtaa
     1441 ctacaatgtt atgtctgtta taccctcgat cccgggaatc acttccccaa attatgtgtt
     1501 tgagtcgacc gccgtaaaag gccgcgtctt ctcggatgaa gctgtgaaag agctagagcg
     1561 ggaagcatcc gaagccgtca agaagcttgc cagatttaaa tcacttaccg acaagaactg
     1621 ggctgatgat tatgactccg atgaggatta cggtctggag agagaggctg caacaaatgc
     1681 gcccgcagag aaaactgctc aaacaaactc agcagagaag actgctccat caacttcagc
     1741 agagaaaact gctctaacaa acaagccttt aaatgggcaa gcggcaccgt ccgccaaaac
     1801 aaacggcaac tccgacatcc ccgacgccgc tacaagcgca ccaccaatgg acaaaatggt
     1861 cgaacagatc atcacagcta tggtggggag aatcaatctc tcggagatag aggagaagat
     1921 agtgagcagg gtgtctcaga aagccctgca gaagcccaaa caaaagaagc gcggaaggcg
     1981 tggagggaag aacaagcaaa acagtttacc tcctacttca acgcaatcta caagtggggc
     2041 gcccaagaag gaggctgccc cccaggcttc aggaagtgcg ggcacatccc gcgctactac
     2101 cacccccgca ccagaggcga aacccagtgg gggcaaaaac tctgccaagt tcaccccgag
     2161 ctggcggata aaacagcagg attcggctgg ccaaaagccg gatctgaagc tgaactccaa
     2221 agcctgaatc tacaggctgc caggtggctc caacgcgcgg agtcagccac tatccctggc
     2281 gcggaagcaa gaaagcgcgt gattgagaaa acagttgagg catacagaaa ttgtgtaact
     2341 aacgccccac tgtgctccct taaatccaaa ctggattggg ctggctttca acaagatatc
     2401 cgtgaagcag tccagtccct tgagctagac gctggtgtag gcattcccta tatcgcgtat
     2461 ggcctccccg cacaccgagg atgggttgag gaccataagc ttctcccagt gctcactcag
     2521 ctgacctttg accgactaca gaagatgtcg gaggccagct ttgaggatat gagcgcagaa
     2581 gagctagttc aagaagggct ctgtgatcct atcagactat ttgtcaaagg agagccccac
     2641 aaacagagca aactcgatga aggccgctac cgcctcatca tgtctgtttc cttggtggat
     2701 caactggtag cccgggttct gttccaaaat cagaacaaaa gggaaatttc cctgtggagg
     2761 tctgtgcctt ccaaacccgg ttttggcctt tcaactgaca ctcaaactgc tgaattcttg
     2821 gagtgtcttc aaaaggtgtc tggagcgcca tctgtggaag aattgtgtgc aaatcacaag
     2881 gagtacacgc gcccaactga ctgttccggt ttcgactggt cagtcgcgta ttggatgctg
     2941 gaggatgata tggaggtgag aaatcgcctg acatttaata acacccagct caccaagcgt
     3001 cttcgggctg cctggttgaa gtgcatagga aactccgtcc tgtgcctgtc cgatggcact
     3061 ttacttgccc aaactgttcc cggtgtgcaa aagagcggaa gttacaatac aagttcctcc
     3121 aactctagaa tccgggttat ggctgcctat cactgtggcg ccgactgggc aatggccatg
     3181 ggggatgatg ctctcgaagc ccccaactcc gacctggagg agtataaaac actaggtttc
     3241 aaagtcgagg taggtcgaga actcgaattc tgttcacaca tcttcagaaa tccgaccctc
     3301 gccgttccgg tcaacaccaa caaaatgctt tacaagttga tccatggtta taatccggaa
     3361 tgtggcaatc cagaagtgat tcaaaactat ctggctgcag tattctctgt gctgcaggaa
     3421 ctccgacacg atcgtgagct cgttgccaag ctccaccagt ggttggttcc gagtgccacc
     3481 acaaaagaac actgaaggag ctcactaaaa ctagccaagc atacacgagt tgcaagcatt
     3541 ggaagttcaa gcctcgttac atcaaccgga ctaaatagat tataaattct tagcgggatt
     3601 tgctttagga ttctcatccg caatcccatt ttcagtagcc ggtttatatt ttgtttacct
     3661 aaagatttcc tcccacgtgc gatcaattgt taatgagtac ggtcgtggtt aaaggaaatg
     3721 tcaatggtgg tgtacaacaa ccaagaatgc gaagaaggca atcccttcgc aggcgcgcta
     3781 acagagttca gccagtggtt atggtcacgg cccctgggca acccaggcgc cgaagacgca
     3841 gaagaggagg caatcgccgc tcaagaagaa ctggagttcc ccgaggacga ggctcaagcg
     3901 agacattcgt gtttacaaag gacaacctcg tgggcaacac ccaaggaagt ttcaccttcg
     3961 ggccgagtct atcagactgt ccggcattca aggatggaat actcaaggcc taccatgagt
     4021 ataagatcac aagcatctta cttcagttcg tcagcgaggc ctcttccacc tcctccggtt
     4081 ccatcgctta tgagttggac ccccattgca aagtatcatc cctccagtcc tacgtcaaca
     4141 agttccaaat tacgaagggc ggcgccaaaa cttatcaagc gcggatgata aacggggtag
     4201 aatggcacga ttcttctgag gatcagtgcc ggatactgtg gaagggaaat ggaaaatctt
     4261 cagattccgc aggatccttc agagtcacca tcaaggtagc tttgcaaaac cccaaatagg
     4321 tagactccgg atcagagcct agtccaagcc cacaaccaac acccactcca actccccaga
     4381 agcacgagcg atttattgct tacgttggca tacctatgct aaccattcag gccagggaga
     4441 acgacgacca aatcatattg ggttccttag ggagccaaag gatgaaatat atagaggacg
     4501 agaaccagaa ttatacaaag tttagttctg agtattactc tcaatcgagc atgcaagccg
     4561 tccctatgta ttacttcaat gtcccgaaag ggcaatggtc agtcgacatc agctgcgaag
     4621 ggtatcaacc cactagcagc acctcggatc caaaccgggg taggagtgac gggatgatcg
     4681 cgtattcaaa cgcggattcc gattattgga atgttggtga agcggatggt gtcaaaattt
     4741 cgaagctacg caacgataac acctaccgcc aaggtcaccc agaacttgaa attaactcgt
     4801 gtcattttcg cgagggtcaa ctccttgaac gggacgctac aattagcttc cacgttgaag
     4861 cgcctactga tgggcgattc ttccttgttg gtcctgccat ccagaaaacc gcaaagtata
     4921 actatactat ctcatacggt gactggacgg accgagacat ggaactgggg ctgatcaccg
     4981 tggtgcttga tgaacattta gaaggcactg gttcggctaa cagagtgcgg cggcccccac
     5041 gggagggcca cacctatatg gcctcgccgc acgaaccgga aggaaaaccg gttggaaata
     5101 aaccaaggga cgaaaccccg atacaaacgc aggaaagaca acctgatcaa actccgtctg
     5161 acgacgtatc cgatgctggt tcggtaaaca gcggcggccc aactgagtcg ctgcgattgg
     5221 agttcggggt aaactcagat agtacctacg atgctacagt cgatggtaca gactggccca
     5281 gaattcctcc accaaggcac ccacctgaac ctagagtttc cggcaattca agaactgtca
     5341 ctgacttttc ttcgaaagcc gatctattgg agaattggga tgccgaacac ttcgaccctg
     5401 gttattccaa agaagatgtc gctgctgcta ctattatagc gcacggcagt attcaagatg
     5461 ggcgaagtat gttggagaag agagaggaaa atgtcaagaa caaaacctcc tcctggaagc
     5521 ccccgtcact taaagcggtg agcccagcca tagccaaatt gcgctcgatt cgcaaatccc
     5581 aaccccttga gggagggacc cttaataaag acgccactga tggtgtctca tctattggca
     5641 gtggttctct aacaggtggc acgcttaaga ggaaggcaac tattgaagaa cgtttactgc
     5701 agaccttaac aactgaacaa aggctgtggt acgagaattt taagaaaact aaccctccag
     5761 ctgctaccca atggctgttt gaatatcagc cacctcccca agtagataga aacatagctg
     5821 aaaagccatt ccaagggagg aaatgagtcg actcacgact taaaactgag tgtccgccgg
     5881 acattaagcg gaacgaaagc cgaaaggtga ttaggctctc aacgtctgct agagaccgtc
     5941 gaaagacgcg actgtgtagc caggatcctc ttacagggtt gtgtagt
//
